Basic Information

Strain Name
C57BL/6N-Dll3tm1(DLL3)/Bcgen
Common Name
B-hDLL3 mice
Background
C57BL/6N
Catalog number
110926
Aliases
DLL3, SCDO1, delta like canonical Notch ligand 3
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hDLL3 mice.

The exons 2-7 of mouse Dll3 gene that encode the extracellular domain were replaced by human DLL3 exons 2-7 in B-hDLL3 mice.

mRNA expression analysis

Strain specific analysis of DLL3 gene expression in wild type (WT) mice and B-hDLL3 mice by RT-PCR.

Mouse Dll3 mRNA was detectable in testis of WT mice (+/+). Human DLL3 mRNA was detectable in homozygous B-hDLL3 mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific DLL3 expression analysis in homozygous B-hDLL3 mice by western blot.

Testis and lung were collected from wild type (WT) mice (+/+) and homozygous B-hDLL3 mice (H/H), and analyzed by western blot with anti-DLL3 antibody. DLL3 was detectable in WT mice (+/+) and homozygous B-hDLL3 mice (H/H) due to the cross-reactivity of antibodies.

Summary

mRNA expression analysis:

Mouse Dll3 mRNA was detectable in testis of WT mice (+/+). Human DLL3 mRNA was detectable in homozygous B-hDLL3 mice (H/H) but not in WT mice (+/+).

Protein expression analysis:

DLL3 was detectable in testis and lung of WT mice (+/+) and homozygous B-hDLL3 mice (H/H) due to the cross-reactivity of antibodies.