Basic Information

Strain Name
C57BL/6Ebi3tm1(EBI3)Bcgen/Bcgen
Common Name
B-hEBI3 mice
Background
C57BL/6
Catalog number
110764
Aliases
EBI3 (IL-27B, IL27B, IL35B)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hEBI3 mice. The exons 1-5 of mouse Ebi3 gene that encode the full-length protein were replaced by human EBI3 exons 1-5 in B-hEBI3 mice.

mRNA and protein expression analysis

Strain specific analysis of EBI3 gene expression in wild-type mice and B-hEBI3 mice by RT-PCR. Mouse Ebi3 mRNA was detectable in splenocytes of wild-type mice (+/+). Human EBI3 mRNA was detectable only in homozygous B-hEBI3 mice (H/H) but not in wild-type mice.

Strain specific EBI3 expression analysis in homozygous B-hEBI3 mice by western blot. Spleen, Kidney, small intestine and muscle tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEBI3 mice (H/H), and analyzed by western blot with anti-EBI3 antibody. Human EBI3 was detectable in wild-type mice and homozygous B-hEBI3 mice, as the antibody is crossly reactive with EBI3 in human and mice.

Summary

mRNA and protein expression analysis:

Human EBI3 mRNA was detectable in B-hEBI3 mice, and we proved that the protein could be expressed normally by western blot analysis.

Back to top