Basic Information
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Targeting strategy
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Gene targeting strategy for B-hEPCAM mice.
The exons 3-8 of mouse Epcam genes that encode the extracellular protein were replaced by human EPCAM exons 2-7 in B-hEPCAM mice.
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Protein expression analysis
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Strain specific EPCAM expression analysis in homozygous B-hEPCAM mice by flow cytometry. Lung, small intestine and pancreas were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEPCAM mice (H/H), and CD45- cells were analyzed by flow cytometry with species-specific anti-EPCAM antibody. Mouse EPCAM was detectable in wild-type mice. Human EPCAM was exclusively detectable in homozygous B-hEPCAM (H/H) but not in wild-type mice.
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Summary
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Protein expression analysis:
Human EPCAM was exclusively detectable in lung, small intestine and pancreas of homozygous B-hEPCAM mice (H/H) but not in wild-type mice, and mouse EPCAM was detectable in wild-type mice.
