Basic Information

Strain Name
C57BL/6-Epcamtm1(EPCAM)Bcgen/Bcgen
Common Name
B-hEPCAM mice
Background
C57BL/6
Catalog number
110826
Aliases
EPCAM (Ber-Ep4, BerEp4, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, MOC-31, TACSTD1, TROP1)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hEPCAM mice. The exons 3-8 of mouse Epcam genes that encode the extracellular protein were replaced by human EPCAM exons 2-7 in B-hEPCAM mice.

Protein expression analysis

Strain specific EPCAM expression analysis in homozygous B-hEPCAM mice by flow cytometry. Lung, small intestine and pancreas were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hEPCAM mice (H/H), and CD45- cells were analyzed by flow cytometry with species-specific anti-EPCAM antibody. Mouse EPCAM was detectable in wild-type mice. Human EPCAM was exclusively detectable in homozygous B-hEPCAM (H/H) but not in wild-type mice.

Publication

EpCAM-targeting CAR-T cell immunotherapy is safe and efficacious for epithelial tumors

Summary

Protein expression analysis:

Human EPCAM was exclusively detectable in lung, small intestine and pancreas of homozygous B-hEPCAM mice (H/H) but not in wild-type mice, and mouse EPCAM was detectable in wild-type mice.

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