Basic Information

Strain Name
C57BL/6-Fgl1tm1(FGL1)/Bcgen
Stock Number
110116
Common Name
B-hFGL1 mice
Background
C57BL/6J
Aliases
Fibrinogen-like protein 1 (FGL-1)
Targeting Strategy
The exons 2~8 of mouse Fgl1 gene that encode the full-length protein were replaced by human FGL1 exons 2~8 in B-hFGL1 mice.
NCBI Gene ID

Details

mRNA expression analysis

Strain specific analysis of FGL1 gene expression in WT and heterozygous B-hFGL1 mice by RT-PCR. Mouse Fgl1 mRNA was detectable in liver cells of wild-type (+/+) mice and heterozygous B-hFGL1 mice. Human FGL1 mRNA was detectable only in H/+, but not in +/+ mice.

 

Protein expression analysis

Strain specific analysis of FGL1 expression in WT and B-hFGL1 mice by western blot. Liver were collected from WT mice and heterozygous B-hFGL1 (H/+) mice. Mouse FGL1 was detectable in WT mice and heterozygous B-hFGL1. Human FGL1 was exclusively detectable in heterozygous B-hFGL1 but not WT mice.