Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFOLR1/hFOLR2 mice. The exons 3~6 and 3’UTR of mouse Folr1 gene and the exons 2~5 of mouse Folr2 gene that encode the full-length were replaced by the exons 3-6 and 3’UTR of human FOLR1 gene and the exons 2~5 of human FOLR2 gene in B-hFOLR1/hFOLR2 mice.
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mRNA expression analysis
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Strain specific FOLR1 and FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow RT-PCR.
Mouse Folr1 and Folr2 mRNA were detectable in splenocytes of wild-type (+/+) mice. Human FOLR1 and FOLR2 mRNA were detectable only in homozygous B-hFOLR1/hFOLR2 mice, but not in wild-type (+/+) mice.
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Protein expression analysis
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Strain specific FOLR1 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by western blot. Kidney, spleen and brain were collected from C57BL/6 mice (+/+) and homozygous B-hFOLR1/hFOLR2 mice (H/H), and analyzed by western blot with anti-FOLR1 antibody. FOLR1 was detectable in kidney and brain but not spleen from wild-type mice and homozygous B-hFOLR1/hFOLR2 mice, as the antibody is crossly reactive with human and mouse FOLR1.
Strain specific FOLR2 expression analysis in wild-type mice and homozygous B-hFOLR1/hFOLR2 mice by flow cytometry. Blood was collected from C57BL/6 mice (+/+) and homozygous B-hFOLR1/hFOLR2 mice (H/H), and analyzed by flow cytometry with anti-FOLR2 antibody. hFOLR2 was only detectable in granulocytes, monocytes and macrophages of homozygous B-hFOLR1/hFOLR2 mice but not in wild-type mice.
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Summary
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mRNA expression analysis
Mouse Folr1 and Folr2 mRNA were detectable in splenocytes of wild-type (+/+) mice. Human FOLR1 and FOLR2 mRNA were detectable only in homozygous B-hFOLR1/hFOLR2 mice, but not in wild-type (+/+) mice.
Protein expression analysis
FOLR1 was detectable in kidney and brain but not spleen from wild-type mice and homozygous B-hFOLR1/hFOLR2 mice, as the antibody is crossly reactive with human and mouse FOLR1. hFOLR2 was only detectable in granulocytes, monocytes and macrophages of homozygous B-hFOLR1/hFOLR2 mice but not in wild-type mice.
