Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFOLR4 mice. The exons 2-5 of mouse Folr4 gene that encode the full-length protein were replaced by human FOLR4 exons 1-4 in B-hFOLR4 mice.
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mRNA and protein expression analysis
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Strain specific analysis of FOLR4 gene expression in wild-type mice and B-hFOLR4 mice by RT-PCR. Mouse Folr4 mRNA was detectable in splenocyte of wild-type mice (+/+). Human FOLR4 mRNA was detectable only in homozygous B-hFOLR4 mice(H/H) but not in wild-type mice.
Strain specific FOLR4 expression analysis in homozygous B-hFOLR4 mice by western blot. Spleen tissue and Kidney tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFOLR4 mice(H/H), and analyzed by western blot with anti-FOLR4 antibody. Human FOLR4 was detectable in wild-type mice and homozygous B-hFOLR4 mice, as the antibody is crossly reactive with FOLR4 in human and mice.
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Summary
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mRNA and protein expression analysis:
Human FOLR4 mRNA was detectable in B-hFOLR4 mice, and we proved that the protein could be expressed normally by western blot analysis.
