Basic Information
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Targeting strategy
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Gene targeting strategy for B-hGAS6 mice. The exons 1-15 of mouse Gas6 gene that encode the full-length protein were replaced by human GAS6 exons 1-15 in B-hGAS6 mice.
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mRNA expression analysis
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Strain specific analysis of GAS6 gene expression in wild-type C57BL/6 mice and B-hGAS6 mice by RT-PCR. Mouse Gas6 mRNA was detectable only in ovary of wild-type C57BL/6 mice (+/+). Human GAS6 mRNA was detectable only in homozygous B-hGAS6 mice (H/H), but not in wild-type mice.
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Protein expression analysis
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Strain specific GAS6 expression analysis in homozygous B-hGAS6 mice by ELISA. Plasma was collected from wild-type mice (+/+) and homozygous B-hGAS6 mice (H/H), and analyzed by ELISA with species-specific GAS6 ELISA kit. Mouse GAS6 was detectable only in wild-type mice. Human GAS6 was exclusively detectable in homozygous B-hGAS6 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Gas6 mRNA was detectable only in ovary of wild-type C57BL/6 mice (+/+). Human GAS6 mRNA was detectable only in homozygous B-hGAS6 mice (H/H), but not in wild-type mice.
Protein expression analysis:
Mouse GAS6 was detectable only in wild-type mice. Human GAS6 was exclusively detectable in homozygous B-hGAS6 mice but not in wild-type mice.
