Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hGAS6 mice. The exons 1-15 of mouse Gas6 gene that encode the full-length protein were replaced by human GAS6 exons 1-15 in B-hGAS6 mice.
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mRNA expression analysis
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Species-specific GAS6 gene expression analysis in wild-type and humanized B-hGAS6 mice by RT-PCR. Murine Gas6 mRNA was detected in ovarian tissue isolated from wild-type C57BL/6 (+/+) mice, while human GAS6 mRNA was exclusively detected in homozygous B-hGAS6 (H/H) mice.
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Protein expression analysis
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Species-specific GAS6 protein expression analysis in wild-type and humanized B-hGAS6 mice. Plasma was isolated from wild-type (+/+) and homozygous B-hGAS6 (H/H) mice, and analyzed by ELISA using species-specific GAS6 kits. Murine GAS6 protein was detected in wild-type mice, while human GAS6 protein was exclusively detected in B-hGAS6 mice.
