Basic Information
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Gene Targeting Strategy
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The human GPC3 CDS was inserted after exon 3 of the mouse Gpc3 gene in B-hGPC3 mice. This insertion disrupts the endogenous murine Gpc3 gene.
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mRNA Expression Analysis
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Species–specific GPC3 expression analysis in wild-type and humanized B-hGPC3 mice. Kidneys were isolated from wild-type and homozygous B-hGPC3 mice and analyzed by RT-PCR for GPC3 mRNA expression. Human GPC3 mRNA was exclusively detected in B-hGPC3 mice compared to wild-type mice.
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Protein Expression Analysis
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Species–specific GPC3 expression analysis in humanized B-hGPC3 mice. Kidney, heart and brain were collected from wild-type (+/+) and homozygous B-hGPC3 (H/H) mice and analyzed by western blot using an anti-GPC3 antibody. GPC3 was detected in wild-type and B-hGPC3 mice (H/H) due to the cross-reactivity of antibodies.
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Summary
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- mRNA expression analysis:
Mouse GPC3 mRNA was detectable in kidney of WT mice (+/+). Human GPC3 mRNA was exclusively detectable in homozygous B-hGPC3 mice (H/H) mice but not in WT mice (+/+).
- Protein expression analysis:
GPC3 was exclusively detectable in kidney, heart and brain from homozygous B-hGPC3 mice (H/H) and WT mice (+/+) due to the cross-reactivity of antibodies.
