Basic Information

Common name
B-hGPC3 mice
Catalog number
110873
Background
C57BL/6
NCBI Gene ID

Gene Targeting Strategy

The human GPC3 CDS was inserted after exon 3 of the mouse Gpc3 gene in B-hGPC3 mice. This insertion disrupts the endogenous murine Gpc3 gene.

mRNA Expression Analysis

Speciesspecific GPC3 expression analysis in wild-type and humanized B-hGPC3 mice. Kidneys were isolated from wild-type and homozygous B-hGPC3 mice and analyzed by RT-PCR for GPC3 mRNA expression. Human GPC3 mRNA was exclusively detected in B-hGPC3 mice compared to wild-type mice.

Protein Expression Analysis

Speciesspecific GPC3 expression analysis in humanized B-hGPC3 mice. Kidney, heart and brain were collected from wild-type (+/+) and homozygous B-hGPC3 (H/H) mice and analyzed by western blot using an anti-GPC3 antibody. GPC3 was detected in wild-type and B-hGPC3 mice (H/H) due to the cross-reactivity of antibodies.