Basic Information

Strain Name
C57BL/6N-Hcar1tm1(HCAR1)Bcgen/Bcgen
Common Name
B-hHCAR1 mice
Background
C57BL/6N
Catalog number
111919
Aliases
HCAR1 (FKSG80, GPR104, GPR81, HCA1, LACR1, TA-GPCR, TAGPCR)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hHCAR1 mice. The exon 1 of mouse Hcar1 gene that encodes the full-length protein was replaced by human HCAR1 exon 1 in B-hHCAR1 mice.

mRNA expression analysis

Strain specific analysis of HCAR1 gene expression in wild-type C57BL/6 mice and B-hHCAR1 mice by RT-PCR. Mouse Hcar1 mRNA was detectable only in BAT (brown adipose tissue) of wild-type C57BL/6 mice (+/+). Human HCAR1 mRNA was detectable only in homozygous B-hHCAR1 mice (H/H), but not in wild-type mice.

Protein expression analysis

Strain specific HCAR1 expression analysis in homozygous B-hHCAR1 mice by flow cytometry. BAT (brown adipose tissue) was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hHCAR1 mice (H/H), and analyzed by western blot with anti-HCAR1 antibody. HCAR1 was both detectable in wild-type mice and homozygous B-hHCAR1 mice (H/H) due to the cross-reactivity of antibodies.

Summary

mRNA expression analysis:

Mouse Hcar1 mRNA was detectable in BAT of wild-type (+/+). Human HCAR1 mRNA was detectable in B-hHCAR1 mice (H/H) mice but not in wild-type mice.

Protein expression analysis:

HCAR1 was both detectable in wild-type mice and homozygous B-hHCAR1 mice due to the cross-reactivity of antibodies.

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