Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hICAM1 mice. The exons 2-7 of mouse Icam1 that encode the full-length protein, not including signal peptides were replaced by the exons 2-7 of human ICAM1 gene in B-hICAM1 mice.
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Protein expression analysis of ICAM1 in T cells and B cells
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Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry.
T cells and B cells from spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibody. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.
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Protein expression analysis of ICAM1 in NK cells, dendritic cells
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Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry.
NK cells, dendritic cells from spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibody. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.
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Protein expression analysis of ICAM1 in granulocytes,monocytes and macrophages
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Strain specific ICAM1 expression analysis in homozygous B-hICAM1 mice by flow cytometry.
Granulocytes, monocytes and macrophages from spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM1 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM1 antibody. Mouse ICAM1 was detectable in wild-type mice. Human ICAM1 was exclusively detectable in homozygous B-hICAM1 mice but not in wild-type mice.
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Summary
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Protein expression analysis:
Human ICAM1 was detectable on T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes, macrophages in homozygous B-hICAM1 mice but not in wild-type mice, and mouse ICAM1 was detectable only in wild-type mice.
