Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hICAM3 mice. The BAC sequences containing 9 kb upstream of human ICAM3 gene, the whole human ICAM3 genomic sequences and 3 kb downstream of the gene were inserted into mouse Rosa26 locus in B-hICAM3 mice.
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Protein expression analysis of ICAM3 in T and B cells
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Species-specific ICAM3 protein expression analysis in wild-type and humanized B-hICAM3 mice. Spleen-derived T and B cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hICAM3 (H/H) mice and analyzed by flow cytometry using an anti-human ICAM3 antibody. Human ICAM3 protein was detected in B-hICAM3 mice but not in wild-type mice.
Species-specific ICAM3 protein expression analysis in wild-type and humanized B-hICAM3 mice. Blood-derived T and B cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hICAM3 (H/H) mice and analyzed by flow cytometry using an anti-human ICAM3 antibody. Human ICAM3 protein was detected in B-hICAM3 mice but not in wild-type mice.
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Protein expression analysis of ICAM3 in NK cells, dendritic cells, granulocytes, monocytes and macrophages
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Strain specific ICAM3 expression analysis in homozygous B-hICAM3 mice by flow cytometry. NK cells, dendritic cells, granulocytes, monocytes, macrophages from spleen were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hICAM3 mice (H/H), and analyzed by flow cytometry with species-specific anti-ICAM3 antibody. Human ICAM3 was exclusively detectable in homozygous B-hICAM3 mice but not in wild-type mice.
Species-specific ICAM3 protein expression analysis in wild-type and humanized B-hICAM3 mice. NK cells, dendritic cells, granulocytes, monocytes and macrophages were isolated from wild-type C57BL/6 (+/+) and homozygous B-hICAM3 (H/H) mice and analyzed by flow cytometry using an anti-human ICAM3 antibody. Human ICAM3 protein was detected in B-hICAM3 mice but not in wild-type mice.
