Basic Information

Strain Name
C57BL/6-Itga5tm1(ITGA5)/Bcgen
Common Name
B-hITGA5 mice
Background
C57BL/6
Catalog Number
111254
Related Gene
integrin subunit alpha 5, CD49e, FNRA, VLA-5, VLA5A
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hITGA5 mice.

The exons 1-29 of mouse Itga5 gene that encode the extracellular domain were replaced by human ITGA5 exons 1-29 in B-hITGA5 mice.

mRNA expression analysis

Strain specific analysis of ITGA5 gene expression in wild-type mice and B-hITGA5 mice by RT-PCR.

Mouse Itga5 mRNA was detectable in bladder of wild-type mice (+/+). Human ITGA5 mRNA was detectable only in homozygous B-hITGA5 but not in wild-type mice.

Protein expression analysis

Strain specific ITGA5 expression analysis in homozygous B-hITGA5 mice by flow cytometry.

Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGA5 mice (H/H) stimulated with anti-CD3e in vivo (7.5 μg/mice, stimulation for 24 hours, i.p. ), and analyzed by flow cytometry with species-specific anti-ITGA5 antibody. Mouse ITGA5 was exclusively detectable in WT mice. Human ITGA5 were exclusively detectable in homozygous B-hITGA5 but not in wild-type mice.

Strain specific ITGA5 expression analysis in homozygous B-hITGA5 mice by flow cytometry.

Splenocytes were collected from wild-type mice (+/+) and homozygous B-hITGA5 mice (H/H) stimulated with anti-CD3e in vivo (7.5 μg/mice, stimulation for 24 hours, i.p. ), and analyzed by flow cytometry with species-specific anti-ITGA5 antibody. Mouse ITGA5 was exclusively detectable in WT mice. Human ITGA5 were exclusively detectable in homozygous B-hITGA5 but not in wild-type mice.

Summary

mRNA expression

Mouse Itga5 mRNA was detectable in bladder of wild-type mice (+/+). Human ITGA5 mRNA was detectable only in homozygous B-hITGA5 but not in wild-type mice.

Protein expression

Mouse ITGA5 was exclusively detectable in WT mice. Human ITGA5 were exclusively detectable in homozygous B-hITGA5 but not in wild-type mice.