Gene targeting strategy for B-hKLB mice. The exons 1-5 of mouse Klb gene that encode the extracellular domain were replaced by human KLB exons 1-5 in B-hKLB mice.
Protein expression analysis
Strain specific analysis of KLB expression in heterozygous B-hKLB mice by Western blot.
Mouse KLB was detectable both in liver of wild type (+/+) mice and heterozygous B-hKLB mice. Human KLB was detectable in heterozygous B-hKLB mice (H/+) , HepG2 cells, and Hep3B cells but not in +/+ mice. HepG2 and Hep3B cells were used as positive control there.