Basic Information

Strain name
C57BL/6-Tnfsf14tm1(TNFSF14)/Bcgen
Common name
B-hLIGHT mice
Background
C57BL/6
Catalog number
110944
Aliases
TNFSF14 (CD258, HVEML, LIGHT, LTg)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hLIGHT mice. The exons 1-4 of mouse Light gene that encode the extracellular protein were replaced by human LIGHT exons 1-4 in B-hLIGHT mice.

Protein expression analysis

Strain specific LIGHT expression analysis in homozygous B-hLIGHT mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hLIGHT mice (H/H) stimulated with anti-mCD3ε, anti-mCD28 and mouse IL-2 in vitro for 24h, and analyzed by flow cytometry with species-specific anti-LIGHT antibody. Human LIGHT was exclusively detectable on CD8+T cells in homozygous B-hLIGHT but not in wild-type mice.

Summary

Protein expression analysis:

Human LIGHT was exclusively detectable on CD8+ T cells of homozygous B-hLIGHT mice but not in wild-type mice.