Basic Information

Strain name
C57BL/6-Tnfsf14tm1(TNFSF14)/Bcgen
Common name
B-hLIGHT mice
Background
C57BL/6N
Catalog number
110944
Aliases
TNFSF14 (CD258, HVEML, LIGHT, LTg)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hLIGHT mice. The exons 1-4 of mouse Light gene that encode the extracellular protein were replaced by human LIGHT exons 1-4 in B-hLIGHT mice.

mRNA expression analysis

Species-specific LIGHT gene expression analysis in wild-type and humanized B-hLIGHT mice by RT-PCR. Murine Light mRNA was detected in splenocytes from wild-type C57BL/6 (+/+) mice, while human LIGHT mRNA was exclusively detected in homozygous B-hLIGHT (H/H) mice.

Protein expression analysis

Species-specific LIGHT protein expression analysis in humanized B-hLIGHT mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLIGHT (H/H) mice, stimulated with anti-mCD3ε, anti-mCD28 and murine IL-2 for 24h in vitro, and analyzed by flow cytometry using an anti-human LIGHT antibody. Human LIGHT protein was exclusively detected on CD8+T cells from B-hLIGHT mice.

Summary

mRNA expression analysis:

Mouse Light mRNA was detectable in splenocytes of wild-type (+/+). Human LIGHT mRNA was detectable in B-hLIGHT mice (H/H) mice but not in wild-type mice.

Protein expression analysis:

Human LIGHT was exclusively detectable on CD8+ T cells of homozygous B-hLIGHT mice but not in wild-type mice.

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