Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-hLIGHT mice. The exons 1-4 of mouse Light gene that encode the extracellular protein were replaced by human LIGHT exons 1-4 in B-hLIGHT mice.
-
mRNA expression analysis
-
Species-specific LIGHT gene expression analysis in wild-type and humanized B-hLIGHT mice by RT-PCR. Murine Light mRNA was detected in splenocytes from wild-type C57BL/6 (+/+) mice, while human LIGHT mRNA was exclusively detected in homozygous B-hLIGHT (H/H) mice.
-
Protein expression analysis
-
Species-specific LIGHT protein expression analysis in humanized B-hLIGHT mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLIGHT (H/H) mice, stimulated with anti-mCD3ε, anti-mCD28 and murine IL-2 for 24h in vitro, and analyzed by flow cytometry using an anti-human LIGHT antibody. Human LIGHT protein was exclusively detected on CD8+T cells from B-hLIGHT mice.
-
Summary
-
mRNA expression analysis:
Mouse Light mRNA was detectable in splenocytes of wild-type (+/+). Human LIGHT mRNA was detectable in B-hLIGHT mice (H/H) mice but not in wild-type mice.
Protein expression analysis:
Human LIGHT was exclusively detectable on CD8+ T cells of homozygous B-hLIGHT mice but not in wild-type mice.
