Key regulator of transforming growth factor beta-1 (TGFB1) specifically required for microglia function in the nervous system (By similarity). Required for activation of latent TGF-beta-1 in macrophages and microglia: associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGFB1, and regulates integrin-dependent activation of TGF-beta-1 (By similarity). TGF-beta-1 activation mediated by LRRC33/NRROS is highly localized: there is little spreading of TGF-beta-1 activated from one microglial cell to neighboring microglia, suggesting the existence of localized and selective activation of TGF-beta-1 by LRRC33/NRROS (By similarity). Indirectly plays a role in Toll-like receptor (TLR) signaling: ability to inhibit TLR-mediated NF-kappa-B activation and cytokine production is probably a consequence of its role in TGF-beta-1 signaling.
mRNA expression analysis
Strain specific analysis of LRRC33 gene expression in WT and the homozygous B-hLRRC33 mice by RT-PCR.
Mouse Lrrc33 mRNA was detectable in thymus of wild-type (+/+) mice. Human LRRC33 mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific analysis of LRRC33 protein expression in C57BL/6 (+/+) and homozygous B-hLRRC33 (H/H) mice by western blot.
Lung were collected from C57BL/6(+/+) and homozygous B-hLRRC33 (H/H) mice. Human LRRC33 was detectable only in B-hLRRC33 (H/H) mice with species-specific anti-hLRRC33 antibody, LRRC33 was detectable in the lung of the C57BL/6(+/+) and B-hLRRC33 (H/H) mice due to the cross-reactivity of antibodies.