Basic Information
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Targeting strategy
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Gene targeting strategy for B-hLRRC33 mice.
The exons 2~3 of mouse Nrros gene that encode the extracellular domain were replaced by human NRROS exons 2~3 in B-hLRRC33 mice.
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mRNA expression analysis
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Strain specific analysis of LRRC33 gene expression in WT and the homozygous B-hLRRC33 mice by RT-PCR.
Mouse Lrrc33 mRNA was detectable in thymus of wild-type (+/+) mice. Human LRRC33 mRNA was detectable only in H/H, but not in +/+ mice.
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Protein expression analysis
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Strain specific analysis of LRRC33 protein expression in C57BL/6 (+/+) and homozygous B-hLRRC33 (H/H) mice by western blot.
Lung were collected from C57BL/6(+/+) and homozygous B-hLRRC33 (H/H) mice. Human LRRC33 was detectable only in B-hLRRC33 (H/H) mice with species-specific anti-hLRRC33 antibody, LRRC33 was detectable in the lung of the C57BL/6(+/+) and B-hLRRC33 (H/H) mice due to the cross-reactivity of antibodies.
