MARCO (SR-A6; SCARA2)
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hMARCO mice. The exons 3-17 of mouse Marco gene that encode the extracelluar protein were replaced by human MARCO exons 3-17 in B-hMARCO mice.
Protein expression analysis
Species-specific MARCO protein expression analysis in wild-type and humanized B-hMARCO mice. Peritoneal exudate macrophages (PEMs) were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hMARCO (H/+) mice stimulated with thioglycolate broth in vivo and analyzed by flow cytometry using species-specific anti-MARCO antibodies. Murine MARCO protein was detected in wild-type and B-hMARCO mice, while human MARCO protein was detected in B-hMARCO mice.