Basic Information

Strain name
C57BL/6-Mcamtm1(MCAM)/Bcgen
Common name
B-hMCAM mice
Catalog number
111168
Background
C57BL/6N
Aliases
MCAM, CD146, HEMCAM, METCAM, MUC18, MelCAM, melanoma cell adhesion molecule
NCBI Gene ID

Gene Targeting Strategy

Gene targeting strategy for B-hMCAM mice. The exons 2-14 of mouse Mcam gene that encode the extracellular domain and the transmembrane domain were replaced by human MCAM exons 2-14 in B-hMCAM mice.

mRNA Expression Analysis

Strain specific analysis of MCAM gene expression in wild type (WT) mice and B-hMCAM mice by RT-PCR. Mouse Mcam mRNA was detectable only in kidney of WT mice (+/+). Human MCAM mRNA was detectable only in homozygous B-hMCAM mice (H/H) but not in WT mice (+/+).

Protein Expression Analysis

Strain specific MCAM expression analysis in homozygous B-hMCAM mice by flow cytometry. Kidney, small intestine and testis were collected from wild type (WT) mice (+/+) and homozygous B-hMCAM mice (H/H), and analyzed by flow cytometry with species-specific anti-MCAM antibody. Mouse MCAM was detectable in WT mice (+/+). Human MCAM was detectable in homozygous B-hMCAM mice (H/H).

Summary

mRNA expression analysis:

  • Mouse MCAM mRNA was detectable in kidney of WT mice (+/+). Human MCAM mRNA was exclusively detectable in homozygous B-hMCAM mice (H/H) mice but not in WT mice (+/+).

Protein expression analysis:

  • Human MCAM was detectable in kidney, small intestine and testis of homozygous B-hMCAM mice (H/H) mice, and mouse MCAM was detectable only in WT mice (+/+).
Back to top