Basic Information
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Targeting strategy
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The exons 2-14 of mouse Mcam gene that encode the extracellular domain and the transmembrane domain were replaced by human MCAM exons 2-14 in B-hMCAM mice.
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mRNA expression analysis
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Strain specific analysis of MCAM gene expression in wild type (WT) mice and B-hMCAM mice by RT-PCR.
Mouse Mcam mRNA was detectable only in kidney of WT mice (+/+). Human MCAM mRNA was detectable only in homozygous B-hMCAM mice (H/H) but not in WT mice (+/+).
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Protein expression analysis
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Strain specific MCAM expression analysis in homozygous B-hMCAM mice by flow cytometry.
Kidney, small intestine and testis were collected from wild type (WT) mice (+/+) and homozygous B-hMCAM mice (H/H), and analyzed by flow cytometry with species-specific anti-MCAM antibody. Mouse MCAM was detectable in WT mice (+/+). Human MCAM was detectable in homozygous B-hMCAM mice (H/H).
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References
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- Wang Z, Xu Q, Zhang N, et al. CD146, from a melanoma cell adhesion molecule to a signaling receptor[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-15
