Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hMCAM mice. The exons 2-14 of mouse Mcam gene that encode the extracellular domain and the transmembrane domain were replaced by human MCAM exons 2-14 in B-hMCAM mice.
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mRNA Expression Analysis
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Strain specific analysis of MCAM gene expression in wild type (WT) mice and B-hMCAM mice by RT-PCR. Mouse Mcam mRNA was detectable only in kidney of WT mice (+/+). Human MCAM mRNA was detectable only in homozygous B-hMCAM mice (H/H) but not in WT mice (+/+).
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Protein Expression Analysis
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Strain specific MCAM expression analysis in homozygous B-hMCAM mice by flow cytometry. Kidney, small intestine and testis were collected from wild type (WT) mice (+/+) and homozygous B-hMCAM mice (H/H), and analyzed by flow cytometry with species-specific anti-MCAM antibody. Mouse MCAM was detectable in WT mice (+/+). Human MCAM was detectable in homozygous B-hMCAM mice (H/H).
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Summary
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mRNA expression analysis:
- Mouse MCAM mRNA was detectable in kidney of WT mice (+/+). Human MCAM mRNA was exclusively detectable in homozygous B-hMCAM mice (H/H) mice but not in WT mice (+/+).
Protein expression analysis:
- Human MCAM was detectable in kidney, small intestine and testis of homozygous B-hMCAM mice (H/H) mice, and mouse MCAM was detectable only in WT mice (+/+).