Basic Information

Strain name
C57BL/6-Mcamtm1(MCAM)/Bcgen
Common name
B-hMCAM mice
Catalog number
111168
Background
C57BL/6
Aliases
MCAM, CD146, HEMCAM, METCAM, MUC18, MelCAM, melanoma cell adhesion molecule

Description

MCAM (melanoma cell adhesion molecule) was originally identified as a melanoma cell adhesion molecule (MCAM) and highly expressed in many tumors and endothelial cells. However, the evidence that CD146 acts as an adhesion molecule to mediate a homophilic adhesion through the direct interactions between CD146 and itself is still lacking. Recent evidence revealed that CD146 is not merely an adhesion molecule, but also a cellular surface receptor of miscellaneous ligands, including some growth factors and extracellular matrixes. Through the bidirectional interactions with its ligands, CD146 is actively involved in numerous physiological and pathological processes of cells. Overexpression of CD146 can be observed in most of malignancies and is implicated in nearly every step of the development and progression of cancers, especially vascular and lymphatic metastasis. Thus, immunotherapy against CD146 would provide a promising strategy to inhibit metastasis, which accounts for the majority of cancer-associated deaths.

Targeting strategy

The exons 2-14 of mouse Mcam gene that encode the extracellular domain and the transmembrane domain were replaced by human MCAM exons 2-14 in B-hMCAM mice.

mRNA expression analysis

Strain specific analysis of MCAM gene expression in wild type (WT) mice and B-hMCAM mice by RT-PCR.

Mouse Mcam mRNA was detectable only in kidney of WT mice (+/+). Human MCAM mRNA was detectable only in homozygous B-hMCAM mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific MCAM expression analysis in homozygous B-hMCAM mice by flow cytometry.

Kidney, small intestine and testis were collected from wild type (WT) mice (+/+) and homozygous B-hMCAM mice (H/H), and analyzed by flow cytometry with species-specific anti-MCAM antibody. Mouse MCAM was detectable in WT mice (+/+). Human MCAM was detectable in homozygous B-hMCAM mice (H/H).

References

  1. Wang Z, Xu Q, Zhang N, et al. CD146, from a melanoma cell adhesion molecule to a signaling receptor[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-15