Basic Information
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Targeting strategy
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Gene targeting strategy for B-hMUC1 mice.
The mouse Muc1 genome that encodes the full-length protein was replaced by human MUC1 genome in B-hMUC1 mice.
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mRNA expression analysis
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Strain specific analysis of MUC1 gene expression in wild-type C57BL/6 mice and hMUC1 mice by RT-PCR. Mouse Muc1 mRNA was detectable only in splenocytes of wild-type C57BL/6 mice (+/+). Human MUC1 mRNA was detectable only in homozygous B-hMUC1 mice (H/H), but not in wild-type mice.
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Protein expression analysis
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Strain specific MUC1 expression analysis in homozygous B-hMUC1 mice by western blot. Different organ tissues were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hMUC1 mice (H/H), and analyzed by western blot with anti-MUC1 antibody. Mouse MUC1 was detectable in wild-type mice and homozygous B-hMUC1 mice due to the cross-reactivity of antibodies. Human MUC1 was exclusively detectable in homozygous B-hMUC1 mice but not wild-type mice.
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B-hMUC1 MC38 can grow in B-hMUC1 mice
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Subcutaneous homograft tumor growth of B-hMUC1 MC38 cells. B-hMUC1 MC38 cells(2-C07 clone) (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into B-hMUC1 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hMUC1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
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Summary
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mRNA expression analysis
The human MUC1 mRNA were detectable in the liver of homozygous B-hMUC1 mice but not in wild-type mice.
Protein expression analysis
Human MUC1 was exclusively detectable in homozygous B-hMUC1 mice but not wild-type mice.
