Basic Information
Strain Name
C57BL/6-Klrc1tm1(KLRC1)/Bcgen
Stock Number
110041
Common Name
B-hNKG2A mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
NKG2A, KLRC1
Species
C57BL/6N
Appearance
Black
Genotypes
Heterozygous
NCBI Gene ID
-
Targeting strategy
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Gene targeting strategy for B-hNKG2A mice. The exon 2~6 of mouse Klrc1 gene that encode the extracellular domain were replaced by human KLRC1 exon 2~6 in B-hNKG2A mice.
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Protein expression analysis
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Species-specific NKG2A protein expression analysis in humanized B-hNKG2A mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hNKG2A (H/+) mice and analyzed by flow cytometry using species-specific anti-NKG2A antibodies. Murine NKG2A protein was detected in wild-type and heterozygous B-hNKG2A mice, while human NKG2A protein was only detected in B-hNKG2A mice. (Monalizumab was used to detect the human NKG2A protein in WT and heterozygous B-hNKG2A mice)
