Basic Information
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Targeting strategy
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Gene targeting strategy for B-hNRP1 mice.
The exons 1-16 of mouse Nrp1 gene that encode the extracellular domain were replaced by human NRP1 exons 1-16 in B-hNRP1 mice.
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Protein expression analysis in Treg cells
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Strain specific NRP1 expression analysis in homozygous B-hNRP1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNRP1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NRP1 antibody. Mouse NRP1 was detectable in wild-type mice. Human NRP1 was exclusively detectable in homozygous B-hNRP1 but not in wild-type mice.
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Protein expression analysis in CD4+ T cells
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Strain specific NRP1 expression analysis in homozygous B-hNRP1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNRP1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NRP1 antibody. Mouse NRP1 was detectable in wild-type mice. Human NRP1 was exclusively detectable in homozygous B-hNRP1 but not in wild-type mice.
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Summary
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Protein expression analysis:
Human NRP1 was both detectable on CD4+ T cells and Treg cells in homozygous B-hNRP1 mice but not in wild-type mice, and mouse NRP1 was detectable only in wild-type mice.
