Basic Information

Strain Name
C57BL/6-Nrp1tm1(NRP1)/Bcgen
Common Name
B-hNRP1 mice
Background
C57BL/6
Catalog number
111330
Aliases
NRP1 (NP1; NRP; BDCA4; CD304; VEGF165R)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hNRP1 mice. 

The exons 1-16 of mouse Nrp1 gene that encode the extracellular domain were replaced by human NRP1 exons 1-16 in B-hNRP1 mice.

Protein expression analysis in Treg cells

Strain specific NRP1 expression analysis in homozygous B-hNRP1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNRP1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NRP1 antibody. Mouse NRP1 was detectable in wild-type mice. Human NRP1 was exclusively detectable in homozygous B-hNRP1 but not in wild-type mice.

Protein expression analysis in CD4+ T cells

Strain specific NRP1 expression analysis in homozygous B-hNRP1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNRP1 mice (H/H), and analyzed by flow cytometry with species-specific anti-NRP1 antibody. Mouse NRP1 was detectable in wild-type mice. Human NRP1 was exclusively detectable in homozygous B-hNRP1 but not in wild-type mice.

Summary

Protein expression analysis:

Human NRP1 was both detectable on CD4+ T cells and Treg cells in homozygous B-hNRP1 mice but not in wild-type mice, and mouse NRP1 was detectable only in wild-type mice.

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