Basic Information

Strain Name
C57BL/6-Ntrk1tm1(NTRK1)/Bcgen
Stock Number
110810
Common Name
B-hNTRK1 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes

Description

NTRK1 encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer.

Gene description

Gene targeting strategy for B-hNTRK1 mice. The exons 1-10 of mouse Ntrk1 gene that encode the extracellular domain were replaced by human NTRK1 exons 1-10 in B-hNTRK1 mice.

Targeting Strategy

 

 

Gene targeting strategy for B-hNTRK1 mice. The exons 1-10 of mouse Ntrk1 gene that encode the extracellular domain were replaced by human NTRK1 exons 1-10 in B-hNTRK1 mice.

Protein expression analysis

Strain specific NTRK1 expression analysis in homozygous B-hNTRK1 mice by western blotting. Cerebellum tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNTRK1 mice (H/H) and analyzed by western blotting with anti-NTRK1 antibody. Mouse NTRK1 was detectable in wild-type mice and homozygous B-hNTRK1 mice with a known cross-reactive anti-NTRK1 antibody. The detected protein size was 140 kDa, represents a mature form of glycoprotein. Human NTRK1 was also detectable in homozygous B-hNTRK1 mice and wild-type mice with another anti-NTRK1 antibody. So this antibody maybe also a cross-reactive antibody. The detected protein size was about 110 kDa, represents a primary form of glycoprotein.