Basic Information

Strain Name
C57BL/6-Ntrk1tm1(NTRK1)Bcgen/Bcgen
Stock Number
110810
Common Name
B-hNTRK1 mice
Background
C57BL/6
NCBI Gene ID
Aliases
MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Gene targeting strategy

Gene targeting strategy for B-hNTRK1 mice. The exons 1-10 of mouse Ntrk1 gene that encode the extracellular domain were replaced by human NTRK1 exons 1-10 in B-hNTRK1 mice.

mRNA expression analysis

Strain specific analysis of NTRK1 gene expression in wild-type C57BL/6 mice and B-hNTRK1 mice by RT-PCR. Mouse NTRK1 mRNA was detectable only in cerebellum tissue of wild-type mice (+/+). Human NTRK1 mRNA was detectable only in homozygous B-hNTRK1 mice (H/H) but not in wild-type mice.

Protein expression analysis

Strain specific NTRK1 expression analysis in homozygous B-hNTRK1 mice by western blotting. Cerebellum tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNTRK1 mice (H/H) and analyzed by western blotting with anti-NTRK1 antibody. Mouse NTRK1 was detectable in wild-type mice and homozygous B-hNTRK1 mice with a known cross-reactive anti-NTRK1 antibody. The detected protein size was 140 kDa, represents a mature form of glycoprotein. Human NTRK1 was also detectable in homozygous B-hNTRK1 mice and wild-type mice with another anti-NTRK1 antibody, thus this antibody may also be a cross-reactive antibody. The detected protein size was about 110 kDa, represents a primary form of glycoprotein.

Back to top
WordPress Double Opt-in by Forge12