Basic Information
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Targeting Strategy
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Details
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Phenotype
Protein Expression Analysis
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry. Mouse 4-1BB+ T cells were detectable in the WT C57BL/6 mice, while human 4-1BB+ T cells were detectable in the homozygous B-hPD-1/h4-1BB mice.
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/h4-1BB mice were analyzed by flow cytometry. Mouse PD-1+ T cells were detectable in the WT C57BL/6 mice, while human PD-1+ T cells were detectable in the homozygous B-hPD-1/h4-1BB mice.
Application
Combination therapy of PD-1 (Keytruda) mAb and 4-1BB (Urelunab Analog ) mAb
Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPD-1/h4-1BB mice. Mice were grouped (n=5) when the tumor size was approximately 150 ± 50mm3 (n=6). Anti-hPD-1 antibody and anti-h4-1BB antibody significantly inhibited tumor growth in the homozygous B-hPD-1/h4-1BB mice, suggesting that B-hPD-1/h4-1BB mouse model is an effective tool for in vivo efficacy evaluation of hPD-1 and h4-1BB antibody combination therapy. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM
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References
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- Clin Transl Immunology. 2014 Aug 1;3(8): e22. doi: 10.1038/cti.2014.18. eCollection 2014 Aug.
- J Immunol. 2002 Jun 1;168(11):5483-90.
- Nat Commun. 2017 Feb 6; 8: 14369. doi: 10.1038/ncomms14369.
- EMBO J. 1992 Nov;11(11): 3887-95.
- J Exp Med. 2000 Oct 2;192(7): 1027-34.
- Science. 2001 Jan 12;291(5502): 319-22.