Basic Information

Strain Name
C57BL/6-Pdcd1tm1(PDCD1)Cd276tm1(CD276)/Bcgen
Stock Number
121152
Common Name
B-hPD-1/hB7-H3 mice
Background
C57BL/6
Related Genes
PD-1, PDCD1, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2
Targeting Strategy
Exon 2 of the mouse Pd-1 gene (which encodes the extracellular domain) was replaced by human PD-1 exon 2 in B-hPD-1/hB7-H3 mice. Exons 3-4 of mouse Cd276 (which encodes the extracellular domain) was replaced by human CD276 exons 3-6 in B-hPD-1/hB7-H3 mice.
NCBI Gene ID

Details

Protein expression analysis in T cells

Strain specific PD-1 expression analysis in homozygous B-hPD-1/hB7-H3  (H/H) mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hB7-H3  (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Mouse PD-1 was detectable in WT mice. Human PD-1 was exclusively detectable in homozygous B-hPD-1/hB7-H3 but not WT mice.

Strain specific analysis of B7-H3 expression in WT and B-hPD-1/hB7-H3 mice by western blot. Epididymis were collected from WT (+/+) mice and homozygous B-hPD-1/hB7-H3 mice. Mouse B7-H3 was detectable in WT mice. Human B7-H3 was detectable in the epididymis of B-hPD-1/hB7-H3 mice due to the cross-reactivity of antibodies.