Basic Information
Strain Name
C57BL/6-Pdcd1tm1(PDCD1) Cd27tm1(CD27)/Bcgen
Stock Number
120518
Common Name
B-hPD-1/hCD27 Mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
PD-1(Programmed death-1); CD27(CD27 molecule) Species: C57BL/6
Appearance
Black
Genotypes
Homozygous
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Targeting Strategy
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Details
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Phenotype
Protein Expression Analysis
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry. Mouse CD27+ T cells were detectable in both WT C57BL/6 and the homozygous B-hPD-1/hCD27 mice, while human CD27+ T cells were detectable in the homozygous B-hPD-1/hCD27 mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-1/hCD27 mice were analyzed by flow cytometry. Mouse PD-1+ T cells were detectable in the WT C57BL/6 mice, while human PD-1+ T cells were detectable in the homozygous B-hPD-1/hCD27 mice.
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References
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- Blood. 2017 Jul 20;130(3):297-309. doi: 10.1182/blood-2016-12-756585. Epub 2017 May 11.
- J Clin Oncol. 2017 Jun 20;35(18):2028-2036. doi: 10.1200/JCO.2016.70.1508. Epub 2017 May 2.
- Immunotherapy. 2015;7(6):655-67. doi: 10.2217/imt.15.32. Epub 2015 Jun 22.
- Nat Commun. 2017 Feb 6; 8: 14369. doi: 10.1038/ncomms14369.
- EMBO J. 1992 Nov;11(11):3887-95.
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