B-hPD-1/hCD27 mice

Basic Information

Targeting Strategy

 

Gene targeting strategy for B-hPD-1/hCD27 mice . The exon 2 of mouse Pdcd1 gene that encode the extracellular domain were replaced by human PD-1 exon 2, The exons 1-5 of mouse Cd27 gene that encode the extracellular domain were replaced by human CD27 exons 1-5 in B-hPD-1/hCD27 mice.

Details

Phenotype

Protein Expression Analysis

Strain specific CD27 expression analysis in homozygous B-hPD-1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-1/hCD27 mice but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.

Strain specific PD-1 expression analysis in homozygous B-hPD-1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Mouse PD-1 was exclusively detectable in WT mice. Human PD-1 were exclusively detectable in B-hPD-1/hCD27 mice but not WT mice.

References

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