B-hPD-1/hGITR mice

Basic Information

Targeting Strategy

Details

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-PD-1/hGITR mice were analyzed by flow cytometry. Mouse PD-1 + T cells were only detectable in the WT C57BL/6 mice, while human PD-1 + T cells were only detectable in the homozygous B-hPD-1/hGITR mice.

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-PD-1/hGITR mice were analyzed by flow cytometry. Mouse GITR + T cells were only detectable in the WT C57BL/6 mice, while human GITR + T cells were only detectable in the homozygous B-hPD-1/hGITR mice.

Analysis of leukocytes cell subpopulation in B-hPD-1/hGITRmice

Analysis of leukocytes subpopulation in spleen .Splenocytes were isolated from C57BL/6 and B-hPD-1/hGITR mice (n=3). The proportion of leukocytes subpopulation was tested by flow cytometry. As a result, the expression profile of leukocytes subpopulation in homozygous B-hPD-1/hGITR mice is similar to that in the C57BL/6 mice, indicating that differentiation of T, B, NK, Monocyte, DC and macrophage cells are not affected by the humanization of hPD-1/hGITR.

routine test

Blood Routine Test. Blood from the C57BL/6 and B-hPD-1/hGITR mice (n=3) was collected and analyzed by blood routine test. As a result, there is no significant differences between the C57BL/6 and B-hPD-1/hGITR mice.

Blood Biochemical test

Blood Biochemical Test. Serum AST/ALT levels of C57BL/6 and B-hPD-1/hGITR mice were tested (n=3). Results indicate that there is no significant differences between the C57BL/6 and B-hPD-1/hGITR mice.

Combination Therapy of PD-1 Ab and GITR Ab Efficacy Evaluation

Murine colon cancer MC38-hPD-L1 cells were subcutaneously implanted into homozygous B-hPD-1/hGITR mice. Mice were grouped when the tumor size was approximately 100-150 mm3 (n=5). All experimental groups compared with the control group show significant inhibitory effects. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

Combination Therapy of PD-1(Keytruda) Ab and GITR Ab Efficacy Evaluation

Murine colon cancer MC38-hPD-L1 cells were subcutaneously implanted into homozygous B-hPD-1/hGITR mice. Mice were grouped when the tumor size was approximately 100-150 mm3 (n=5). All experimental groups compared with the control group show significant inhibitory effects. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.

References

1. Nat Commun.2017 Feb 6;8:14369. doi: 10.1038/ncomms14369.
2. EMBO J.1992 Nov;11(11):3887-95.
3. ONCOIMMUNOLOGY. 2017, VOL. 6, NO. 3, e1280645 (14 pages). doi: 10.1080/2162402X.2017.1280645