Basic Information

Strain name
C57BL/6-Pdcd1tm1(PDCD1)Cd274tm1(CD274)C5ar1tm1(C5AR1)/Bcgen
Common name
B-hPD-1/hPD-L1/hC5AR1  mice
Background
C57BL/6
Catalog number
131216
Related genes
PDCD1: CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1; CD274: B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1; C5AR1: C5A, C5AR, C5R1, CD88

Target strategy

The exon 2 of mouse PD-1 gene that encode the extracellular domain was replaced by human PD-1 exon 2 in B-hPD-1/hPD-L1/hC5AR1  mice. The exon 3 of mouse Pdl1 gene that encode the extracellular domain was replaced by human PD-L1 exon 3 in B-hPD-1/hPD-L1/hC5AR1  mice. The exon 2 of mouse C5ar1 gene that encodes the full-length protein was replaced by human C5AR1 exon 2 in B-hPD-1/hPD-L1/hC5AR1  mice. This treble knock-in model was developed by breeding the B-hPD-1/hPD-L1 mice and the B-hC5AR1 mice together.

Protein expression analysis

Strain specific PD-1, PD-L1, C5AR1 expression analysis in homozygous B-hPD-1/hPD-L1/hC5AR1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hC5AR1 mice (H/H) for PD-1 and PD-L1 expression analysis; Bone marrow was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hC5AR1 mice (H/H) for C5AR1 expression analysis. Analyses were performed by flow cytometry with species-specific anti-PD-1, anti-PD-L1 and anti-C5AR1 antibody. Mouse PD-1, PD-L1, C5AR1 were detectable in wild-type C57BL/6 mice. Human PD-1, PD-L1, C5AR1 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hC5AR1 mice but not in wild-type mice.