B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice

Basic Information

Strain Name
C57BL/6-Pdcd1tm1(PDCD1) Cd274tm1(CD274) ,Tg(RP11-634C1)1/Bcgen
Common Name
B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice
Background
C57BL/6
Catalog number
111954
Related Genes
PD-1 (Programmed death-1) ; CD274 (CD274 antigen); LILRB2(ILT4, LIR2, CD85D, ILT-4, LIR-2, MIR10, MIR-10); LILRB3(HL9, ILT5, LIR3, PIRB, CD85A, ILT-5, LIR-3, PIR-B, LILRA6);

Analysis of spleen leukocytes

Analysis of spleen leukocyte subpopulations. Splenocytes were isolated from wild-type C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice (female, n=3, 7-week-old) and analyzed by flow cytometry to assess spleen leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45+ cells and used for further analysis as indicated. (B) Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in wild-type mice. Values are expressed as mean ± SEM.

Analysis of spleen T cells

Analysis of spleen T cell subpopulations. Splenocytes were isolated from wild-type C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice (female, n=3, 7-week-old) and analyzed by flow cytometry to assess T cell subpopulations. (A) Representative flow cytometry plots. Single live CD45+ cells were gated on TCRβ+ T cells and used for further analysis as indicated. (B) Percent of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in wild-type mice. Values are expressed as mean ± SEM.