Basic Information
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Analysis of spleen leukocytes
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Analysis of spleen leukocyte subpopulations. Splenocytes were isolated from wild-type C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice (female, n=3, 7-week-old) and analyzed by flow cytometry to assess spleen leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45+ cells and used for further analysis as indicated. (B) Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in wild-type mice. Values are expressed as mean ± SEM.
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Analysis of spleen T cells
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Analysis of spleen T cell subpopulations. Splenocytes were isolated from wild-type C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice (female, n=3, 7-week-old) and analyzed by flow cytometry to assess T cell subpopulations. (A) Representative flow cytometry plots. Single live CD45+ cells were gated on TCRβ+ T cells and used for further analysis as indicated. (B) Percent of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in wild-type mice. Values are expressed as mean ± SEM.
