Basic Information
Strain Name
BALB/c-Pdcd1tm1(PDCD1)/Bcgen
Common Name
B-hPD-1 mice(C)
Background
BALB/c
Catalog number
112015
Related Genes
CD279, PD-1
NCBI Gene ID
-
Gene targeting strategy
-
Gene targeting strategy for B-hPD-1 mice(C). The exon 2 of mouse PD-1 gene that encodes the IgV domain was replaced by human PD-1 exon 2 in B-hPD-1 mice(C).
-
Protein expression analysis
-
Species-specific PD-1 protein expression analysis in wild-type BALB/c and humanized B-hPD-1 mice(C). Splenocytes were isolated from wild-type BALB/c (+/+) and homozygous B-hPD-1 (H/H) mice(C) stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.) and analyzed by flow cytometry using species-specific anti-PD-1 antibodies. Mouse PD-1 protein was detected in wild-type mice, while human PD-1 protein was detected in B-hPD-1 mice(C).
