Basic Information

Strain Name
Common Name
B-hPD-1 mice(C)
Catalog number
Related Genes
CD279, PD-1

Gene targeting strategy

Gene targeting strategy for B-hPD-1 mice(C). The exon 2 of mouse PD-1 gene that encodes the IgV domain was replaced by human PD-1 exon 2 in B-hPD-1 mice(C).

Protein expression analysis

Species-specific PD-1 protein expression analysis in wild-type BALB/c and humanized B-hPD-1 mice(C). Splenocytes were isolated from wild-type BALB/c (+/+) and homozygous B-hPD-1 (H/H) mice(C) stimulated with anti-CD3ε in vivo (7.5 μg/mice for 24 hours, i.p.) and analyzed by flow cytometry using species-specific anti-PD-1 antibodies. Mouse PD-1 protein was detected in wild-type mice, while human PD-1 protein was detected in B-hPD-1 mice(C).

In vivo efficacy of anti-human PD-1 antibody

Antitumor activity of anti-human PD-1 antibody pembrolizumab (in house) in B-hPD-1 mice(C). (A) Anti-human PD-1 antibody inhibited B-hPD-L1 CT26.WT tumor growth in B-hPD-1 mice(C). Murine colon cancer B-hPD-L1 CT26.WT cells were subcutaneously implanted into homozygous B-hPD-1 mice(C) (female, 9-week-old, n=6). Mice were grouped when tumor volume reached approximately 60-80 mm3, at which time they were treated with anti-human PD-1 antibody indicated in panel. (B) Body weight changes during treatment. As shown in panel A, anti-human PD-1 antibody was efficacious in controlling tumor growth in B-hPD-1 mice(C), demonstrating that the B-hPD-1 mice(C) provide a powerful preclinical model for in vivo evaluation of anti-human PD-1 antibodies. Values are expressed as mean ± SEM.


  • Protein expression analysis:

Mouse PD-1 was only detectable in wild-type BALB/c mice. Human PD-1 was only detectable in homozygous B-hPD-1 mice(C).

  • In vivo efficacious:

Anti-human PD-1 antibody were efficacious in controlling tumor growth in B-hPD-1 mice(C).

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