Basic Information

Strain Name
C57BL/6-Cd274tm1(CD274) Cd27tm1(CD27)/Bcgen
Stock Number
120537
Common Name
B-hPD-L1/hCD27 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD27(CD27 molecule); Cd274 (CD274 antigen)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

Targeting Strategy

 

Gene targeting strategy for B-hPD-L1/hCD27 mice .

The exon 3 of mouse Cd274 gene that encode the extracellular domain were replaced by human CD274 exon 3, The exons 1-5  of mouse Cd27 gene that  encode the extracellular domain were replaced by human CD27 exons 1-5  in B-hPD-1/hCD27 mice.

Details

Protein Expression Analysis

 

 

 

 

 

 

 

 

 

 

Strain specific PD-L1 expression analysis in homozygous B-hPD-L1/hCD27  mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was exclusively detectable in WT mice. Human PD-L1 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice.

Strain specific CD27 expression analysis in homozygous B-hPD-L1/hCD27  mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-L1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.

References

  1. Clin Cancer Res 2016; published online Nov 8. DOI:10.1158/1078-0432.CCR-16-1741
  2. Blood First Edition paper, May 11, 2010; DOI 10.1182/blood-2010-02-271874.
  3. Journal of Clinical Oncology 35, no. 18 (June 20 2017) 2028-2036. DOI: 10.1200/JCO.2016.70.1508