Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hPD-L1/hCD27 mice .
The exon 3 of mouse Cd274 gene that encode the extracellular domain were replaced by human CD274 exon 3, The exons 1-5 of mouse Cd27 gene that encode the extracellular domain were replaced by human CD27 exons 1-5 in B-hPD-1/hCD27 mice.
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Details
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Protein Expression Analysis
Strain specific PD-L1 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was exclusively detectable in WT mice. Human PD-L1 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice.
Strain specific CD27 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-L1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.
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References
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- Clin Cancer Res 2016; published online Nov 8. DOI:10.1158/1078-0432.CCR-16-1741
- Blood First Edition paper, May 11, 2010; DOI 10.1182/blood-2010-02-271874.
- Journal of Clinical Oncology 35, no. 18 (June 20 2017) 2028-2036. DOI: 10.1200/JCO.2016.70.1508
