Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hPD-L1/hOX40 mice. The exon 3 of mouse Pd-l1 gene that encodes the extracellular domain was replaced by human PD-L1 exon 3 and the exons 1-5 of mouse Tnfrsf4 gene that encode the extracellular domain were replaced by human TNFRSF4 exons 1-5 in B-hPD-L1/hOX40 mice.
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Details
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Phenotype
Protein Expression Analysis
Combination therapy of PD-L1 Ab and OX40 Ab
Antitumor activity of anti-hOX40 antibody combined with anti-hPD-L1 antibody in B-hPD-L1/hOX40 mice. (A) Anti-hOX40 antibody combined with anti-hPD-L1 antibodies inhibited MC38-hPD-L1 tumor growth in B-hPD-L1/hOX40 mice. Murine colon cancer MC38-hPD-L1 cells (5×105) were subcutaneously implanted into homozygous B-hPD-L1/hOX40 mice (female, 6-7 week-old, n=6). Mice were grouped when tumor volume reached approximately 150±50 mm3, at which time they were treated with anti-hOX40 antibody combined with anti-hPD-L1 antibody with doses and schedules indicated in panel (B) Body weight changes during treatment. As shown in panel A, combination of anti-hOX40 and anti-hPD-L1 antibody shows more inhibitory effects than individual groups, demonstrating that the B-hPD-L1/hOX40 mice provide a powerful preclinical model for in vivo evaluating combination therapy efficacy of hOX40 antibodies and hPD-L1 antibodies. Values are expressed as mean ± SEM.
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References
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- Nat Rev Immunol. 2004 Jun;4(6):420-31.
- Structure. 2006 Aug;14(8):1321-30.
- Immunol Rev. 2011 Nov;244(1):218-31. doi: 10.1111/j.1600-065X.2011.01069.x.