B-hPD-L1/hOX40 Mice

Basic Information

Targeting Strategy

Gene targeting strategy for B-hPD-L1/hOX40 mice. The exon 3 of mouse Pd-l1 gene that  encodes the extracellular domain was replaced by human PD-L1 exon 3 and  the exons 1-5 of mouse Tnfrsf4 gene that  encode the extracellular domain were replaced by human TNFRSF4  exons 1-5 in B-hPD-L1/hOX40 mice.

Details

Phenotype

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-L1/hOX40 mice were analyzed by flow cytometry. Mouse OX40+ T cells were detectable in the WT C57BL/6 mice, while human OX40+ T cells were detectable in the homozygous B-hPD-L1/hOX40 mice.

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hPD-L1/hOX40 mice were analyzed by flow cytometry.Mouse PD-L1+ T cells were detectable in the WT mice, while human PD-L1+ T cells were detectable in the homozygous B-hPD-L1/hOX40 mice.

References

1. Nat Rev Immunol. 2004 Jun;4(6):420-31.
2. Structure. 2006 Aug;14(8):1321-30.
3. Immunol Rev. 2011 Nov;244(1):218-31. doi: 10.1111/j.1600-065X.2011.01069.x.