Gene targeting strategy for B-hPD-L1/hSIRPA/hCD47 mice. The exon 3 of mouse Pdl1 gene that encode the extracellular domain was replaced by human PD-L1 exon 3 in B-hPD-L1/hSIRPA/hCD47 mice. The exon 2 of mouse Sirpα gene that encode the extracellular domain was replaced by human SIRPα exon 2. The exon 2 of mouse Cd47 gene that encode the extracellular domain was replaced by human CD47 exon 2 in the B-hSIRPA/hCD47 mice. This triple knock-in mouse model, was developed by mating the B-hPD-L1 mice, B-hSIRPA mice and B-hCD47 mice together.
Human CD47/PD-L1 bispecific mAb efficacy evaluation
MC38-hPD-L1-hCD47 cells were injected subcutaneously into the B-hPD-L1/hCD47/hSIRPA mice. The treatment was initiated when mean tumor sizes reached approximately 200 mm3.The results indicated that the bispecific antibody showed better efficacy among these antibodies. B-hPD-L1/hCD47/hSIRPA mice model was an effective tool for in vivo hPD-L1/hCD47 antibody efficacy study. (A) Tumor average volume ± SEM, (B) Mice average weight ±SEM.
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