Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hPD-L1 mice(C).
The exon 3 of mouse Pdl1 gene that encodes the IgV domain was replaced by human PD-L1 exon 3 in B-hPD-L1 mice(C).
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Protein expression analysis
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Strain specific PD-L1 expression analysis in wild-type BALB/c mice and heterozygous B-hPD-L1 mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice and heterozygous B-hPD-L1 mice(C) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.). Mouse PD-L1 was both detectable in wild-type mice and heterozygous B-hPD-L1 mice(C). Human PD-L1 was only detectable in heterozygous B-hPD-L1 mice(C).
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Summary
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Protein expression：
Mouse PD-L1 was both detectable in wild-type mice and heterozygous B-hPD-L1 mice(C). Human PD-L1 was only detectable in heterozygous B-hPD-L1 mice(C).
