PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hPD-L1 mice(C). Exon 3 of mouse Pdl1 gene that encodes the IgV domain was replaced by human PD-L1 exon 3 in B-hPD-L1 mice(C).
Protein expression analysis
Species-specific PD-L1 protein expression analysis in wild-type and humanized B-hPD-L1 mice(C). Splenocytes were isolated from wild-type BALB/c (+/+) and heterozygous B-hPD-L1 mice(C) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.). Murine PD-L1 protein was detected in wild-type and heterozygous B-hPD-L1 mice(C), while human PD-L1 protein was only detected in B-hPD-L1 mice(C).