Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hPDGFB mice.
The exons 1-6 of mouse Pdgfb gene that encode the full-length protein were replaced by human PDGFB exons 1-6 in B-hPDGFB mice.
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Protein expression analysis
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Strain specific PDGFB expression analysis in homozygous B-hPDGFB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hPDGFB mice (H/H) and analyzed by ELISA with PDGF BB ELISA kit. Mouse PDGF BB was exclusively detectable in wild-type mice. Human PDGF BB was both detectable in wild-type mice and homozygous B-hPDGFB mice, as the kit is crossly reactive with human and mouse PDGF BB.
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Summary
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Protein expression analysis:
Human PDGF BB was both detectable in serum of homozygous B-hPDGFB mice and wild-type mice, as the kit is crossly reactive with human and mouse PDGF BB. Mouse PDGF BB was detectable only in wild-type mice.
