PDGFB (SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2)
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hPDGFB mice. Exons 1-6 of mouse Pdgfb gene that encode the full-length protein were replaced by human PDGFB exons 1-6 in B-hPDGFB mice.
Protein expression analysis
Species-specific PDGFB protein expression analysis in wild-type and humanized B-hPDGFB mice. Serum was collected from wild-type (+/+) and homozygous B-hPDGFB (H/H) mice and analyzed by ELISA using PDGF-BB ELISA kits. Murine PDGF-BB protein was only detected in wild-type mice, while human PDGF-BB protein was detected in both wild-type and B-hPDGFB mice, due to the cross-reactive kit.