Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPDPN mice. The exons 2-5 of mouse Pdpn gene that encode the extracellular region were replaced by human PDPN exons 2-5 in B-hPDPN mice.
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Protein expression analysis
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Strain specific PDPN expression analysis in homozygous B-hPDPN mice by western blot.
Lung tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPDPN mice (H/H), and analyzed by western blot with anti-PDPN antibody. Mouse PDPN was only detectable in wild-type C57BL/6 mice. Human PDPN was only detectable in homozygous B-hPDPN mice but not in wild type mice.
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mRNA expression analysis
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Strain specific analysis of PDPN gene expression in wild-type C57BL/6 mice and B-hPDPN mice by RT-PCR. Mouse Pdpn mRNA was detectable in brain of wild-type C57BL/6 mice (+/+). Human PDPN mRNA was detectable only in homozygous B-hPDPN mice (H/H) but not in wild type mice.
