Basic Information

Strain name
C57BL/6-Pdpntm1(PDPN)/Bcgen
Common name
B-hPDPN mice
Background
C57BL/6
Catalog number
111099
Aliases
AGGRUS, GP36, GP40, Gp38, HT1A-1, OTS8, PA2.26, T1A, T1A-2, T1A2, TI1A
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hPDPN mice. The exons 2-5 of mouse Pdpn gene that encode the extracellular region were replaced by human PDPN exons 2-5 in B-hPDPN mice.

Protein expression analysis

Strain specific PDPN expression analysis in homozygous B-hPDPN mice by western blot.

Lung tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPDPN mice (H/H), and analyzed by western blot with anti-PDPN antibody. Mouse PDPN was only detectable in wild-type C57BL/6 mice. Human PDPN was only detectable in homozygous B-hPDPN mice but not in wild type mice.

mRNA expression analysis

Strain specific analysis of PDPN gene expression in wild-type C57BL/6 mice and B-hPDPN mice by RT-PCR. Mouse Pdpn mRNA was detectable in brain of wild-type C57BL/6 mice (+/+). Human PDPN mRNA was detectable only in homozygous B-hPDPN mice (H/H) but not in wild type mice.