Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPLXNB2 mice.
The exons 4~23 of mouse Plxnb2 gene that encode the extracellular domain were replaced by human PLXNB2 exons 3~22 in B-hPLXNB2 mice. -
Protein expression analysis in DC cells
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Strain specific PLXNB2 expression analysis in homozygous B-hPLXNB2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPLXNB2 mice (H/H), and analyzed by flow cytometry with species-specific anti-PLXNB2 antibody. mPLXNB2 was detectable in wild-type mice, while hPLXNB2 was only detectable in homozygous mice.
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Protein expression analysis in monocytes
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Strain specific PLXNB2 expression analysis in homozygous B-hPLXNB2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPLXNB2 mice (H/H), and analyzed by flow cytometry with species-specific anti-PLXNB2 antibody. mPLXNB2 was detectable in wild-type mice, while hPLXNB2 was only detectable in homozygous mice.
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Protein expression analysis in macrophages
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Strain specific PLXNB2 expression analysis in homozygous B-hPLXNB2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPLXNB2 mice (H/H), and analyzed by flow cytometry with species-specific anti-PLXNB2 antibody. mPLXNB2 was detectable in wild-type mice, while hPLXNB2 was only detectable in homozygous mice.
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Protein expression analysis in granulocytes
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Strain specific PLXNB2 expression analysis in homozygous B-hPLXNB2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPLXNB2 mice (H/H), and analyzed by flow cytometry with species-specific anti-PLXNB2 antibody. mPLXNB2 was not detectable in granulocytes of wild-type mice, and hPLXNB2 was not detectable in granulocytes of homozygous mice.
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Summary
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Protein expression analysis:
Human PLXNB2 was detectable on monocytes, macrophages and DC cells in homozygous B-hPLXNB2 mice, while hPLXNB2 was not detectable on granulocytes of homozygous mice.
Mouse PLXNB2 was detectable on monocytes, macrophages and DC cells in wild-type C57BL/6 mice, while mPLXNB2 was not detectable on granulocytes of wild-type C57BL/6 mice.
