Basic Information
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Targeting strategy
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Gene targeting strategy for B-hRAGE mice. The exons 1~11 of mouse Rage gene that encode the full-length protein were replaced by human RAGE exons 1~11 in B-hRAGE mice.
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mRNA expression analysis
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Strain specific analysis of RAGE gene expression in WT and B-hRAGE mice by RT-PCR. Mouse Rage mRNA was detectable in splenocytes of wild-type (+/+) mice. Human RAGE mRNA was detectable only in H/H, but not in +/+ mice.
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Protein expression analysis
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Western blot analysis of RAGE protein expression in homozygous B-hRAGE mice. Lung, spleen, and brain tissue lysates were collected from wild-type C57BL/6N mice (+/+), homozygous B-hRAGE mice (H/H), and then analyzed by western blot with species-specific anti-RAGE antibody(R&D, MAB11451). 40 μg total proteins were loaded for western blotting analysis. RAGE was detected in lung.
