Basic Information

Strain Name
C57BL/6-Agertm1(AGER)/Bcgen
Stock Number
111107
Common Name
B-hRAGE mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
AGER (Advanced glycosylation end-product specific receptor), RAGE, SCARJ1
Species
C57BL/6N
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hRAGE mice. The exon 1~11 of mouse Rage gene that encode the full-length protein were replaced by human RAGE exon 1~11 in B-hRAGE mice.

Details

mRNA expression analysis

Strain specific analysis of RAGE gene expression in WT and B-hRAGE mice by RT-PCR. Mouse Rage mRNA was detectable in splenocytes of wild-type (+/+) mice. Human RAGE mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis

Strain specific analysis of RAGE expression in WT and B-hRAGE mice by western blot. Lung were collected from WT mice and homozygous B-hRAGE (H/H) mice. Mouse RAGE was detectable in WT mice. Human RAGE was exclusively detectable in homozygous B-hRAGE but not WT mice.