Gene targeting strategy for B-hRAGE mice. The exon 1~11 of mouse Rage gene that encode the full-length protein were replaced by human RAGE exon 1~11 in B-hRAGE mice.
mRNA expression analysis
Strain specific analysis of RAGE gene expression in WT and B-hRAGE mice by RT-PCR. Mouse Rage mRNA was detectable in splenocytes of wild-type (+/+) mice. Human RAGE mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific analysis of RAGE expression in WT and B-hRAGE mice by western blot. Lung were collected from WT mice and homozygous B-hRAGE (H/H) mice. Mouse RAGE was detectable in WT mice. Human RAGE was exclusively detectable in homozygous B-hRAGE but not WT mice.