Basic Information

Strain Name
C57BL/6-Tnfsf11tm1(TNFSF11)/Bcgen
Stock Number
110772
Common Name
B-hRANKL mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf
Species
C57BL/6
Appearance
Black
Genotypes
Heterozygous

Description

RANKL encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy.

Details

Protein expression analysis in CD4+ T cells

Strain specific RANKL expression analysis in heterozygous B-hRANKL mice by flow cytometry. CD4+ T cells were isolated from the splenocytes of wild-type C57BL/6 (+/+) mice and heterozygous B-hRANKL mice (H/+) with negative selection on microbeads. Mouse RANKL was detectable both in WT mice and in heterozygous B-hRANKL mice. Human RANKL was exclusively detectable in heterozygous B-hRANKL mice.

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