Basic Information

Strain Name
C57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen
Common Name
B-hSEMA4D mice
Background
C57BL/6
Catalog number
112334
Aliases
SEMA4D (A8, GR3, BB18, CD100, COLL4, SEMAJ, coll-4, C9orf164, M-sema-G)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hSEMA4D mice.

The chimeric SEMA4D CDS (replace the extracellular domain) was inserted in the exon 4 of mouse Sema4d gene in B-hSEMA4D mice.

Protein expression analysis (T cells)

Strain specific SEMA4D expression analysis in T cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (B cells)

Strain specific SEMA4D expression analysis in B cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (NK cells)

Strain specific SEMA4D expression analysis in NK cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (DC cells)

Strain specific SEMA4D expression analysis in DC cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (granulocytes)

Strain specific SEMA4D expression analysis in granulocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (macrophages)

Strain specific SEMA4D expression analysis in macrophages of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (monocytes)

Strain specific SEMA4D expression analysis in monocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Summary

Protein expression analysis:

Human SEMA4D was both detectable on T cells, B cells, NK cells, DC cells, monocytes, macrophages and granulocytes in homozygous B-hSEMA4D mice but not in wild-type mice, and mouse SEMA4D was detectable only in wild-type mice.

Back to top