Basic Information
Description
Sialic acid-binding Ig-like lectin 10 is a protein that in humans is encoded by the SIGLEC10 gene. Siglec-g is often referred to as the murine paralog of human Siglec-10. Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immuno-receptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24, CD52 (the target of the therapeutic monoclonal antibody alemtuzumab) and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10. Tumor-expressed CD24 in promoting immune evasion through its interaction with the inhibitory receptor sialic acid-binding Ig-like lectin-10, which is expressed by tumor-associated macrophages.
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Details
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Protein expression analysis in B cells
Strain specific SIGLEC10 expression analysis in heterozygous B-hSIGLEC10 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hSIGLEC10 (H/+) mice, and analyzed by flow cytometry with species-specific SIGLEC10 antibody. Mouse SIGLEC10 was detectable in WT mice and heterozygous B-hSIGLEC10. Human SIGLEC10 was exclusively detectable in heterozygous B-hSIGLEC10 but not WT mice.
Protein expression analysis in macrophages
Strain specific SIGLEC10 expression analysis in heterozygous B-hSIGLEC10 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hSIGLEC10 (H/+) mice, and analyzed by flow cytometry with species-specific SIGLEC10 antibody. Mouse SIGLEC10 was detectable in WT mice and heterozygous B-hSIGLEC10. Human SIGLEC10 was exclusively detectable in heterozygous B-hSIGLEC10 but not WT mice.
