Basic Information

Strain name
C57BL/6-Scarb1tm1(SCARB1)/Bcgen
Common name
B-hSRB1 mice
Background
C57BL/6
Catalog number
111115
Aliases
SCARB1, CD36L1, CLA-1, CLA1, HDLQTL6, SR-BI, SRB1, scavenger receptor class B member 1
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hSRB1 mice.

The exons 2-11 of mouse Scarb1 gene that encode the extracellular domain and partial transmembrane domain of  were replaced by human SCARB1 exons 2-7 in B-hSRB1 mice.

mRNA expression analysis

Strain specific analysis of SRB1 gene expression in wild type (WT) mice and B-hSRB1 mice by RT-PCR.

Mouse Srb1 mRNA was detectable in liver of WT mice (+/+). Human SRB1 mRNA was exclusively detectable in homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific SRB1 expression analysis in homozygous B-hSRB1 mice by western blot.

Liver was collected from wild type (WT) mice (+/+) and homozygous B-hSRB1 mice (H/H), and analyzed by western blot with species-specific anti-SRB1 antibody. SRB1 was detected in WT mice (+/+), homozygous B-hSRB1 mice (H/H), positive 293T cells and HepG2 cells when an anti-mSRB1 antibody recognizing the intracellular region of mouse SRB1 was used. Human SRB1 was exclusively detectable in homozygous B-hSRB1 mice (H/H).

Summary

mRNA expression analysis:

Mouse SRB1 mRNA was detectable in liver of WT mice (+/+). Human SRB1 mRNA was exclusively detectable in homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+).

Protein expression analysis:

Human SRB1 was exclusively detectable in liver of homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+). SRB1 was detected in WT mice (+/+), homozygous B-hSRB1 mice (H/H), positive 293T cells and HepG2 cells when an anti-mSRB1 antibody recognizing the intracellular region of mouse SRB1 was used.