Basic Information
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Targeting strategy
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Gene targeting strategy for B-hSRB1 mice.
The exons 2-11 of mouse Scarb1 gene that encode the extracellular domain and partial transmembrane domain of were replaced by human SCARB1 exons 2-7 in B-hSRB1 mice.
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mRNA expression analysis
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Strain specific analysis of SRB1 gene expression in wild type (WT) mice and B-hSRB1 mice by RT-PCR.
Mouse Srb1 mRNA was detectable in liver of WT mice (+/+). Human SRB1 mRNA was exclusively detectable in homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+).
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Protein expression analysis
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Strain specific SRB1 expression analysis in homozygous B-hSRB1 mice by western blot.
Liver was collected from wild type (WT) mice (+/+) and homozygous B-hSRB1 mice (H/H), and analyzed by western blot with species-specific anti-SRB1 antibody. SRB1 was detected in WT mice (+/+), homozygous B-hSRB1 mice (H/H), positive 293T cells and HepG2 cells when an anti-mSRB1 antibody recognizing the intracellular region of mouse SRB1 was used. Human SRB1 was exclusively detectable in homozygous B-hSRB1 mice (H/H).
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Summary
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mRNA expression analysis:
Mouse SRB1 mRNA was detectable in liver of WT mice (+/+). Human SRB1 mRNA was exclusively detectable in homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+).
Protein expression analysis:
Human SRB1 was exclusively detectable in liver of homozygous B-hSRB1 mice (H/H) but not in WT mice (+/+). SRB1 was detected in WT mice (+/+), homozygous B-hSRB1 mice (H/H), positive 293T cells and HepG2 cells when an anti-mSRB1 antibody recognizing the intracellular region of mouse SRB1 was used.
