Basic Information
-
Gene targeting strategy
-
Gene targeting strategy for B-hTIGIT mice(C).
The exon 2 of mouse Tigit gene that encodes the extracellular region was replaced by human TIGIT exon 2 in B-hTIGIT mice(C).
-
Protein expression analysis
-
Strain specific TIGIT expression analysis in homozygous B-hTIGIT mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and homozygous B-hTIGIT mice(C) (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TIGIT antibody. Mouse TIGIT was detectable on CD4+ T cells and CD8+ T cells of wild-type mice. Human TIGIT was exclusively detectable on CD4+ T cells and CD8+ T cells of homozygous B-hTIGIT mice(C) but not in wild-type mice.
Strain specific TIGIT expression analysis in homozygous B-hTIGIT mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and homozygous B-hTIGIT mice(C) (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TIGIT antibody. Mouse TIGIT was detectable on Tregs and NK cells of wild-type mice. Human TIGIT was exclusively detectable on Treg cells and NK cells of homozygous B-hTIGIT mice(C) but not in wild-type mice.
-
Summary
-
Protein expression analysis:
Human TIGIT was exclusively detectable on CD4+ T cells, CD8+ T cells, NK cells and Tregs of homozygous B-hTIGIT mice(C) but not in wild-type mice.
