Basic Information

Strain Name
BALB/c-Tigittm1(TIGIT)/Bcgen
Common Name
B-hTIGIT mice(C)
Background
BALB/c
Catalog number
112252
Aliases
VSIG9, VSTM3, WUCAM
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hTIGIT mice(C).

The exon 2 of mouse Tigit gene that encodes the extracellular region was replaced by human TIGIT exon 2 in B-hTIGIT mice(C).

Protein expression analysis

Strain specific TIGIT expression analysis in homozygous B-hTIGIT mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and homozygous B-hTIGIT mice(C) (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TIGIT antibody. Mouse TIGIT was detectable on CD4+ T cells and CD8+ T cells of wild-type mice. Human TIGIT was exclusively detectable on CD4+ T cells and CD8+ T cells of homozygous B-hTIGIT mice(C) but not in wild-type mice.

Strain specific TIGIT expression analysis in homozygous B-hTIGIT mice(C) by flow cytometry. Splenocytes were collected from wild-type BALB/c mice (+/+) and homozygous B-hTIGIT mice(C) (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TIGIT antibody. Mouse TIGIT was detectable on Tregs and NK cells of wild-type mice. Human TIGIT was exclusively detectable on Treg cells and NK cells of homozygous B-hTIGIT mice(C) but not in wild-type mice.

Summary

Protein expression analysis:

Human TIGIT was exclusively detectable on CD4+ T cells, CD8+ T cells, NK cells and Tregs of homozygous B-hTIGIT mice(C) but not in wild-type mice.