B-hTLR2 mice

Basic Information

Targeting strategy

Gene targeting strategy for B-hTLR2 mice. The exon 3 of mouse Tlr2 gene that encodes the extracellular domain was replaced by human TLR2 exon 1 in B-hTLR2 mice.

Protein expression analysis in Gr-1+ cells in spleen

Species-specific TLR2 protein expression analysis in humanized B-hTLR2 mice. Peritoneal exudate macrophages (PEMs) were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hTLR2 (H/+) mice, and analyzed by flow cytometry using species-specific anti-TLR2 antibodies. Murine TLR2 protein was detected in wild-type and heterozygous B-hTLR2 mice, while human TLR2 protein was exclusively detected in B-hTLR2 mice.