Basic Information

Strain Name
C57BL/6-Tlr8tm1(TLR8)/Bcgen
Common Name
B-hTLR8 mice
Background
C57BL/6
Catalog Number
110104
Aliases
toll-like receptor 8, CD288

Description

Toll-like receptor 8 is a protein that in humans is encoded by the TLR8 gene and a member of the toll-like receptor (TLR) family, also called CD288 (cluster of differentiation 288). This gene is predominantly expressed in lung and peripheral blood leukocytes, and lies in close proximity to another family member, TLR7, on chromosome X. TLR8 can recognize GU-rich single-stranded RNA. However, the presence of GU-rich sequences in the single-stranded RNA is not sufficient to stimulate TLR8. TLR8 recognizes G-rich oligonucleotides. Furthermore, TLR8 is an endosomal receptor that recognizes single stranded RNA (ssRNA), and can recognize ssRNA viruses such as Influenza, Sendai, and Coxsackie B viruses. TLR8 binding to the viral RNA recruits MyD88 and leads to activation of the transcription factor NF-κB and an antiviral response. TLR8 agonists (e.g. VTX-2337) have undergone clinical trials as immune stimulants in combination therapy for some cancers.

Details

Protein expression analysis in T cells

Strain specific TLR8 expression analysis in homozygous B-hTLR8 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT mice and homozygous B-hTLR8 due to the anti-mouse TLR8 antibody cross-reacts with human TLR8. Human TLR8 was exclusively detectable in homozygous B-hTLR8 but not WT mice.

Protein expression analysis in DC cells

Strain specific TLR8 expression analysis in homozygous B-hTLR8 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT mice and homozygous B-hTLR8 due to the anti-mouse TLR8 antibody cross-reacts with human TLR8. Human TLR8 was exclusively detectable in homozygous B-hTLR8 but not WT mice.

Protein expression analysis in DC cells

Strain specific TLR8 expression analysis in homozygous B-hTLR8 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT mice and homozygous B-hTLR8 due to the anti-mouse TLR8 antibody cross-reacts with human TLR8. Human TLR8 was exclusively detectable in homozygous B-hTLR8 but not WT mice.

Protein expression analysis in monocytes

Strain specific TLR8 expression analysis in homozygous B-hTLR8 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT mice and homozygous B-hTLR8 due to the anti-mouse TLR8 antibody cross-reacts with human TLR8. Human TLR8 was exclusively detectable in homozygous B-hTLR8 but not WT mice.

Protein expression analysis in monocytes

Strain specific TLR8 expression analysis in homozygous B-hTLR8 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT mice and homozygous B-hTLR8 due to the anti-mouse TLR8 antibody cross-reacts with human TLR8. Human TLR8 was exclusively detectable in homozygous B-hTLR8 but not WT mice.

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