Basic Information

Strain Name
C57BL/6-Tlr8tm1(TLR8)/Bcgen
Common Name
B-hTLR8 mice
Background
C57BL/6J
Catalog Number
110104
Aliases
toll-like receptor 8, CD288
NCBI Gene ID

Expression analysis in dendritic cells

Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans.. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.

Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans.. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.

Expression analysis in monocytes

Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.

Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.

Expression analysis in T cells

Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.

Functional analysis using TLR8 agonists

Functional assay using homozygous B-hTLR8 mice. TNFα, IFNγ and IL12p40 are important downstream cytokines in the NF-κb and IRF7 signaling pathways. Splenocytes were isolated from wild-type (+/+) and homozygous B-hTLR8 (H/H) mice, stimulated with TLR8 agonists TLR8-506 and GS-9688 (Selgantolimod) in vitro, and analyzed using murine TNFα, IFNγ and IL12p40 ELISA kits. TNFα, IFNγ and IL12p40 cytokine secretion increased in B-hTLR8 mice compared to wild-type mice due to the high binding affinity to human TLR8. Lastly, stimulation with GS-9688 showed a dose-dependent effect on cytokine secretion.

Poster

AACR 2022: Generation of a Novel Humanized TLR8 Mouse Model for the Evaluation of Human TLR8 Agonists and Antibody-Conjugated TLR8 Agonists