Basic Information
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Expression analysis in dendritic cells
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Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans.. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.
Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans.. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.
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Expression analysis in monocytes
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Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.
Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.
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Expression analysis in T cells
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Splenocytes were collected from WT and homozygous B-hTLR8 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TLR8 antibody. Mouse TLR8 was detectable in WT and homozygous B-hTLR8 mice compared to isotype due to the cross-reactivity of the anti-mTLR8 antibody between mice and humans. Human TLR8 was exclusively detectable in homozygous B-hTLR8 mice compared to WT.
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Functional analysis using TLR8 agonists
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Functional assay using homozygous B-hTLR8 mice. TNFα, IFNγ and IL12p40 are important downstream cytokines in the NF-κb and IRF7 signaling pathways. Splenocytes were isolated from wild-type (+/+) and homozygous B-hTLR8 (H/H) mice, stimulated with TLR8 agonists TLR8-506 and GS-9688 (Selgantolimod) in vitro, and analyzed using murine TNFα, IFNγ and IL12p40 ELISA kits. TNFα, IFNγ and IL12p40 cytokine secretion increased in B-hTLR8 mice compared to wild-type mice due to the high binding affinity to human TLR8. Lastly, stimulation with GS-9688 showed a dose-dependent effect on cytokine secretion.
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Poster
