Basic Information

Strain Name
C57BL/6-Tnfrsf1btm1(TNFRSF1B) Tnfrsf1atm1(TNFRSF1A) /Bcgen
Stock Number
120802
Common Name
B-hTNFR2/hTNFR1 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
TNFRSF1B (Tumor necrosis factor receptor superfamily, member 1b); TNFRSF1A (Tumor necrosis factor receptor superfamily, member 1a)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

mRNA expression analysis

Strain specific analysis of TNFR1 and TNFR2 gene expression in WT and B-hTNFR2/TNFR1 mice by RT-PCR. Mouse Tnfr1 and Tnfr2 mRNA were detectable only in splenocytes of wild-type (+/+) mice. Human TNFR1 and TNFR2 mRNA were detectable only in H/H, but not in +/+ mice.

Protein expression analysis

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2/hTNFR1 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2/hTNFR1 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2/hTNFR1 but not WT mice.

Protein expression analysis-granulocytes

Strain specific TNFR1 expression analysis in homozygous B-hTNFR2/hTNFR1 mice by flow cytometry. Splenocytes and blood were collected from WT and homozygous B-hTNFR2/hTNFR1 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR1 antibody. Mouse TNFR1 was detectable in the WT mice. Human TNFR1 was exclusively detectable in homozygous B-hTNFR2/hTNFR1 but not WT mice.