Basic Information
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mRNA Expression Analysis
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Species-specific gene expression analysis of TNFR1 and TNFR2 in wild-type and B-hTNFR2/TNFR1 mice. Murine Tnfr1 and Tnfr2 mRNA was detected in splenocytes from wild-type (+/+) mice, while human TNFR1 and TNFR2 mRNA was exclusively detected in homozygous B-hTNFR2/hTNFR1 mice compared to wild-type mice.
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Protein Expression Analysis
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Species-specific TNFR2 expression analysis in homozygous B-hTNFR2/hTNFR1 mice. Following anti-CD3ε stimulation in vivo, splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hTNFR2/hTNFR1 (H/H) mice, and analyzed by flow cytometry using species-specific anti-TNFR2 antibodies. Murine TNFR2 protein was detected in wild-type mice, while human TNFR2 protein was exclusively detected in homozygous B-hTNFR2/hTNFR1 mice.
Species-specific TNFR1 expression analysis in homozygous B-hTNFR2/hTNFR1 mice. Splenocytes and blood were collected from wild-type C57BL/6 (+/+) and homozygous B-hTNFR2/hTNFR1 (H/H) mice, and analyzed by flow cytometry using species-specific anti-TNFR1 antibodies. Murine TNFR1 protein was detected in the wild-type mice, while human TNFR1 protein was exclusively detected in homozygous B-hTNFR2/hTNFR1 mice.