Basic Information

Strain Name
C57BL/6-Tnfrsf1btm1(hTNFRSF1B)/Bcgen
Common Name
B-hTNFR2 mice
Background
C57BL/6
Product No
110032
Related Gene
TNFRSF1B (Tumor necrosis factor receptor superfamily, member 1b)

Description

TNFR2 is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2 (TRAF2), which mediates anti-apoptotic signals.

Details

mRNA expression analysis

Strain specific analysis of TNFR2 gene expression in WT and hTNFR2 mice by RT-PCR. Mouse Tnfr2 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human TNFR2 mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 but not WT mice.

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 but not WT mice.

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 but not WT mice.

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 but not WT mice.

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 but not WT mice.

Strain specific TNFR2 expression analysis in homozygous B-hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFR2 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFR2 but not WT mice.

Back to top