Basic Information

Common Name
B-hTREM1 mice
Catalog Number
111180
Background
C57BL/6N
NCBI Gene ID

Gene Targeting Strategy

Exons 1~4 of the murine Trem1 gene, which encode the extracellular domain, were replaced by human TREM1 exons 1~4 in B-hTREM1 mice.

mRNA Expression Analysis

TREM1 gene expression analysis in wild type and humanized B-hTREM1 mice. Human TREM1 mRNA expression level in B-hTREM1 (H/H) mice was similar to murine Trem1 expression level in C57BL/6 (+/+), demonstrating that introduction of hTREM1 in place of its mouse counterpart does not change the overall expression level of TREM1 mRNA.

Protein Expression Analysis

Species-specific TREM1 expression analysis in humanized B-hTREM1 mice. Blood was collected from wild-type C57BL/6 (+/+) and heterozygous B-hTREM1 (H/+) mice and analyzed by flow cytometry using species-specific anti-TREM1 antibodies. Mouse TREM1 was detected in wild-type and heterozygous B-hTREM1 mice, while human TREM1 was exclusively detected in humanized B-hTREM1 mice.

Tumor Growth Curve and Body Weight Changes

Subcutaneous homograft tumor growth of MC38 cells in B-hTREM1 mice. Wild-type murine colon cancer MC38 cells (5×105) were subcutaneously implanted into wild-type C57BL/6 mice and humanized B-hTREM1 mice (male, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. Shown in panel A, MC38 cells were able to establish tumors in B-hTREM1 mice, which can be used for in vivo efficacy studies.