Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hTREM2 mice(C). Exons 1~5 and 3` UTR of the mouse Trem2 gene that encode the full-length protein were replaced by human TREM2 exons 1~5 and 3` UTR in B-hTREM2 mice(C).
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mRNA expression analysis
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Species-specific TREM2 gene expression analysis in wild-type and humanized B-hTREM2 mice(C) by RT-PCR. Murine Trem2 mRNA was detected in wild-type BALB/c (+/+) mice, while human TREM2 mRNA was detected in homozygous B-hTREM2 (H/H) mice(C).
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Protein expression analysis
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Species-specific TREM2 protein expression analysis in wild-type and humanized B-hTREM2 mice(C). Microglial cells were isolated from wild-type BALB/c (+/+) and homozygous B-hTREM2 (H/H) mice(C) and analyzed by flow cytometry using a cross-reactive anti-TREM2 antibody. TREM2 protein was detected in wild-type and B-hTREM2 mice(C).
