Basic Information
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Targeting strategy
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Gene targeting strategy for B-hTYRO3 mice.
The exons 2~10 of mouse Tyro3 gene that encode the extracellular domain were replaced by human TYRO3 exons 2~10 in B-hTYRO3 mice.
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mRNA expression analysis
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Strain specific analysis of TYRO3 mRNA expression in wild-type C57BL/6 mice and B-hTYRO3 mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTYRO3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TYRO3 primers. Mouse Tyro3 mRNA was detectable only in wild-type C57BL/6 mice. Human TYRO3 mRNA was detectable only in homozygous B-hTYRO3 mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of TYRO3 protein expression in homozygous B-hTYRO3 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTYRO3 mice (H/H), and then analyzed by proteinsimple-western blot with anti-TYRO3 antibody. 3μg total proteins were loaded for western blotting analysis. TYRO3 protein was detected in the lung, stomach and brain of wild-type C57BL/6 mice and homozygous B-hTYRO3 mice, with high expression in the brain, as evidenced by cross-reactivity of antibody against mouse and human TYRO3 proteins.
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Summary
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mRNA expression analysis:
Mouse tyro3 mRNA was detectable in brain of wild-type C57BL/6 mice. Human TYRO3 mRNA was detectable in B-hTYRO3 mice.
Protein expression analysis:
TYRO3 protein was detected in the lung, stomach and brain of wild-type C57BL/6 mice and homozygous B-hTYRO3 mice, with high expression in the brain, as evidenced by cross-reactivity of antibody against mouse and human TYRO3 proteins.
