Basic Information

Strain Name
C57BL/6-Vsirtm2(VSIR)/Bcgen
Stock Number
110049
Common Name
B-hVSIR mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

Details

mRNA expression analysis

Strain specific analysis of VSIR gene expression in WT and hVSIR mice by RT-PCR. Mouse vsir mRNA was detectable in bone marrow cell of wild-type (+/+) mice and heterozygous (H/+) mice. Human VSIR mRNA was detectable only in H/+, but not in +/+ mice.

Protein expression analysis

Strain specific VSIR expression analysis in homozygous B-hVSIR mice by flow cytometry. Bone marrow and blood were collected from WT and homozygous B-hVSIR (H/H) mice in vivo, and analyzed by flow cytometry with species-specific anti-VSIR antibody. Mouse VSIR was detectable in WT mice. Human VSIR was exclusively detectable in homozygous B-hVSIR but not WT mice.