mRNA expression analysis
Strain specific analysis of VSIR gene expression in WT and hVSIR mice by RT-PCR. Mouse vsir mRNA was detectable in bone marrow cell of wild-type (+/+) mice and heterozygous (H/+) mice. Human VSIR mRNA was detectable only in H/+, but not in +/+ mice.
Protein expression analysis
Strain specific VSIR expression analysis in homozygous B-hVSIR mice by flow cytometry. Bone marrow and blood were collected from WT and homozygous B-hVSIR (H/H) mice in vivo, and analyzed by flow cytometry with species-specific anti-VSIR antibody. Mouse VSIR was detectable in WT mice. Human VSIR was exclusively detectable in homozygous B-hVSIR but not WT mice.