Basic Information
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Protein expression analysis (LILRB2)
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Protein expression analysis (F3)
Strain specific LILRB2 expression analysis in transgenic B-Tg(hLILRB2/hLILRB3) mice by flow cytometry. T cells and B cells of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB2/hLILRB3) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB2 antibody. Human LILRB2 was not detectable in T cells of B-Tg(hLILRB2/hLILRB3) mice but detectable in B cells.
Strain specific LILRB2 expression analysis in transgenic B-Tg(hLILRB2/hLILRB3) mice by flow cytometry. Dendritic cells and macrophages of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB2/hLILRB3) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB2 antibody. Human LILRB2 was both detectable in dendritic cells and macrophages from B-Tg(hLILRB2/hLILRB3) mice but not wild-type mice.
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Protein expression analysis (LILRB3)
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Protein expression analysis (F3)
Strain specific LILRB3 expression analysis in transgenic B-Tg(hLILRB2/hLILRB3) mice by flow cytometry. T cells and B cells of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB2/hLILRB3) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB3 antibody. Human LILRB3 was both detectable in T cells and B cells of B-Tg(hLILRB2/hLILRB3) mice but not in wild-type mice.
Strain specific LILRB3 expression analysis in transgenic B-Tg(hLILRB2/hLILRB3) mice by flow cytometry. Dendritic cells and macrophages of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB2/hLILRB3) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB3 antibody. Human LILRB3 was both detectable in dendritic cells and macrophages from B-Tg(hLILRB2/hLILRB3) mice but not wild-type mice.
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In vivo efficacy of anti-human LILRB2 antibody
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Antitumor activity of anti-human LILRB2 antibody in B-Tg(hLILRB2/hLILRB3) mice. (A) Anti-human LILRB2 antibody inhibited MC38 tumor growth in B-Tg(hLILRB2/hLILRB3) mice. Murine colon cancer MC38 cells were subcutaneously implanted into B-Tg(hLILRB2/hLILRB3) mice (female, 9 week-old, n=6). Mice were grouped when tumor volume reached approximately 100-150 mm3, and treated with anti-h LILRB2 antibody in panel A. (B) Body weight changes during treatment. As shown in panel A, J19.h7 (in house) was efficacious in controlling tumor growth in B-Tg(hLILRB2/hLILRB3) mice, demonstrating they provide a powerful preclinical model for in vivo evaluation of anti-human LILRB2 antibodies. Values are expressed as mean ± SEM.
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Summary
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LILRB2 protein expression:
1.Detectable in dendritic cells derived from bone marrow. (F2)
2.Detectable in B cells, dendritic cells and macrophages from blood. (F3)
LILRB3 protein expression:
1.Detectable in dendritic cells derived from bone marrow. (F2)
2.Detectable in T cells, B cells, dendritic cells and macrophages from blood. (F3)
In vivo efficacy of anti-human LILRB2 antibody
Anti-hLILRB2 antibody J19.h7 (in house) was efficacious in controlling tumor growth in B-Tg(hLILRB2/hLILRB3) mice.
